Insertion junction: CLIP2.065596_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.065596
Chromosome:chromosome 14
Location:1087329
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g801609 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTACCCTCTAAATCCCAGTCAACAAATTGTCACGTGCGAAGGGAGTGCG
Internal bar code:AGGGTCAGTAAAGACATATGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2638
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:ATGATGATGATGAGGGCGCC
Suggested primer 2:AGACGCGGTGAAGTTGAGAG