| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.065597 |
| Chromosome: | chromosome 13 |
| Location: | 4999515 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g606050 | TSY1 | Tyrosyl-tRNA synthetase; (1 of 1) PTHR11766//PTHR11766:SF0 - TYROSYL-TRNA SYNTHETASE // TYROSINE--TRNA LIGASE, MITOCHONDRIAL | 3'UTR |
| Cre13.g606101 | (1 of 2) PTHR19959//PTHR19959:SF147 - KINESIN LIGHT CHAIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCTTACACAGCGCCACACGCTCAAGGCGCGCGAGGGGCTGGGGCCAC |
| Internal bar code: | ATGTATATCTGTTCACGTAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2318 |
| LEAP-Seq percent confirming: | 90.4762 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGGACACAGAGTTCAGC |
| Suggested primer 2: | TTTCCCTACCCACTTTCCGC |