| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.065602 |
| Chromosome: | chromosome 12 |
| Location: | 3482777 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497100 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGGACCGGTCCGCCCAGACCTTCGCATGGATGCCCCAACCATGCGGCT |
| Internal bar code: | TAGGGCACGGCAGCATACTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 709 |
| LEAP-Seq percent confirming: | 91.4286 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGTCTACCGAACTCTCCT |
| Suggested primer 2: | TGTGAATCTGAAAGGGCGCT |