| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.065621 |
| Chromosome: | chromosome 11 |
| Location: | 3431521 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g477300 | PPP36,FAP8 | Flagellar Associated Protein 8; (1 of 1) K03456 - serine/threonine-protein phosphatase 2A regulatory subunit A (PPP2R1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTTGGTAGCTGAAGTCAAAAGGCGGGCCTGTCAGGGAGGAGGTTGTGT |
| Internal bar code: | TGTATGCAATGTGTGCGGACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2097 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGTGGTGCTGGTTGGAAA |
| Suggested primer 2: | CGGCATCAATACGGCAACTG |