| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.065648 |
| Chromosome: | chromosome 3 |
| Location: | 3898615 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g170650 | (1 of 1) IPR001849//IPR011993 - Pleckstrin homology domain // Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB) | 3'UTR | |
| Cre03.g170700 | (1 of 3) 3.2.1.39 - Glucan endo-1,3-beta-D-glucosidase / Laminarinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTCAGGGCTCAACGCCTACCGCATGCTTGCCATGCATGTCATACACTT |
| Internal bar code: | AGGCGGCCTATTGTATCGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6437 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTCGACTGGGTTTTGC |
| Suggested primer 2: | CGTGATGGATGGAGCCGTAA |