| Insertion cassette: | CIB2 | 
| Side of cassette: | 3' truncated? | 
| Strand: | + | 
| Strain: | CLIP2.065656 | 
| Chromosome: | chromosome 1 | 
| Location: | 2565527 | 
| Confidence (%): | 80 | 
| Locus systematic id | Locus common name | Defline | Feature | 
|---|---|---|---|
| Cre01.g015103 | Solute-binding protein-like adenylate cyclase; (1 of 7) PF00211//PF13416 - Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // Bacterial extracellular solute-binding protein (SBP_bac_8) | 5'UTR | |
| Cre01.g015150 | 3'UTR | 
| Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTAGCAGGCTTCTGAGCTCATAAACCGTGCACGTGCCCCATCTCTCAT | 
| Internal bar code: | CTCGCTCTGGGCCCGGTTGTAC | 
| Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 933 | 
| LEAP-Seq percent confirming: | 94.7368 | 
| LEAP-Seq n confirming: | 18 | 
| LEAP-Seq n nonconfirming: | 1 | 
| LEAP-Seq n unique pos: | 19 | 
| Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGCCTATATTGCGGTCT | 
| Suggested primer 2: | ATTTCCACGCCTGCACCATA |