| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.065666 |
| Chromosome: | chromosome 13 |
| Location: | 1472156 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g801520 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCCGCGGAGACCTAGCCGCGGCGCATAGACGTAGCCAGGTGATTGCAG |
| Internal bar code: | AGGCATGTGATCGCTGCCAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4641 |
| LEAP-Seq percent confirming: | 6.66667 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 140 |
| LEAP-Seq n unique pos: | 150 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTGGCGCATGATGCTAAC |
| Suggested primer 2: | TGCTGTGACAGGACATGGAC |