| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.065667 |
| Chromosome: | chromosome 16 |
| Location: | 4249787 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g669550 | METC,METC1 | Cystathionine beta-lyase; (1 of 1) 4.4.1.8 - Cystathionine beta-lyase / Cystine lyase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCTAACAAGTATAGGTTGATAAGAAGGTATGGAATAGTTTACCGACAG |
| Internal bar code: | AGCTCGGCGGGCAGCAGGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1048 |
| LEAP-Seq percent confirming: | 42.0455 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCCACCTCTCATCCCTCA |
| Suggested primer 2: | TCAACACGAAACCCTAGCCC |