| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.065673 |
| Chromosome: | chromosome 17 |
| Location: | 5207203 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g737050 | (1 of 1) PTHR22957//PTHR22957:SF287 - TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN // SUBFAMILY NOT NAMED | 3'UTR | |
| Cre17.g737084 | (1 of 1) PTHR15863//PTHR15863:SF2 - FAMILY NOT NAMED // UPF0544 PROTEIN C5ORF45 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGGTAACAAGCGCCCAGTGAACACCCAAGCGCTGCCCCTCGTCATTTC |
| Internal bar code: | CCTGCTAGAATTAAGAGTTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5264 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 107 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 107 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCGATGGTCTGTTTGTGA |
| Suggested primer 2: | GAATCCGAGAGCTCCGATGG |