| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.065677 |
| Chromosome: | chromosome 13 |
| Location: | 1357897 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g571560 | (1 of 1) PF09737 - De-etiolated protein 1 Det1 (Det1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCAAAAGCCCGTCACGAGCTCGGTAACTACCTCAGTGGCCATAGGTGT |
| Internal bar code: | CTCCAGCTTACTCAATCAACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2088 |
| LEAP-Seq percent confirming: | 92.5 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCTGCTTTGGTTCGTCT |
| Suggested primer 2: | CCGAAGGCTTTGAAACTGCC |