Insertion junction: CLIP2.065686_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.065686
Chromosome:chromosome 7
Location:5918169
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre07.g354100 HAT4 (1 of 1) K07739 - elongator complex protein 3 [EC:2.3.1.48] (ELP3, KAT9); Histone acetyltransferase 4 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGCAATACCCGACCCCGCAGCATGGCACGTGTAGGCGCGAGCGGCTTTG
Internal bar code:CTAGCATTCATCTTTTTTCACA

Confirmation - LEAP-Seq

LEAP-Seq distance:166
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GAGACGTGGACCTCAACCAG
Suggested primer 2:TACCCCGTGTACGACTGAGT