| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.065717 |
| Chromosome: | chromosome 6 |
| Location: | 846110 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g255650 | EZY1,EZY25,EZY1B | Copy B in the EZY1 tandem gene cluster | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCGGTTGAGTGCTATAAACCCCTCGGCAAGGCCCATGCAGCCCGCCAT |
| Internal bar code: | AACGCGCGCGTGTACATGTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1619 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGCTGGAGTGGGTGATTG |
| Suggested primer 2: | TGTTGCGCTGATGTCGTAGT |