| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.065721 |
| Chromosome: | chromosome 12 |
| Location: | 8570824 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548800 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCGGGGTGGCGGGTCGGGAGCACAACGCGCGAGCGCGAGCTGCCCAAC |
| Internal bar code: | CTTAGAGGACTTATATAATCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 302 |
| LEAP-Seq percent confirming: | 27.451 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTCAAGTTTGTCGCGTT |
| Suggested primer 2: | GCCACCACCCCATCCAAATA |