Insertion junction: CLIP2.065730_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.065730
Chromosome:chromosome 4
Location:3349754
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre04.g227350 CCR4 Putative NOT-complex component; (1 of 1) K12580 - CCR4-NOT transcription complex subunit 3 (CNOT3, NOT3) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCGCCTTGGCCGCTGCCGCTGCCGCGCGCTCCCGCTCTCGCTCCTTTT
Internal bar code:GGTGAAGGGCGTGCGCAAGACT

Confirmation - LEAP-Seq

LEAP-Seq distance:368
LEAP-Seq percent confirming:60.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CAACCGTTTTGCTGTGCGTA
Suggested primer 2:CAGCTTCAGGTTGGTGTTGC