| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.065733 |
| Chromosome: | chromosome 16 |
| Location: | 5606340 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681351 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGAAATGCAAGTTAAAGAAATGCAAGGACAGATTCACATACCCCATC |
| Internal bar code: | TCGCCAAGACGCGTAGGAAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1443 |
| LEAP-Seq percent confirming: | 97.7273 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTTATGCGAGGCCTAGCG |
| Suggested primer 2: | CTGGCCTACTATGCAGCTCC |