| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.065744 |
| Chromosome: | chromosome 7 |
| Location: | 215219 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313500 | NUP214 | Nucleoporin 214 | 3'UTR_intron |
| Cre07.g313550 | (1 of 1) PTHR15495//PTHR15495:SF17 - NEGATIVE REGULATOR OF VESICLE FORMATION-RELATED // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGATCATGCATGCTGGGCCTCGATTCGCTGGTTAGGATGGAGCTGCAG |
| Internal bar code: | TGCCTTTTGCGGTTGGGTGCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1457 |
| LEAP-Seq percent confirming: | 98.3871 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGATCAGTCGGTGGTGTG |
| Suggested primer 2: | CAAACACACACGCACGCATA |