| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.065753 |
| Chromosome: | chromosome 7 |
| Location: | 4664295 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344800 | TNP21,CSB33 | (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon); {"Probable transposon-derived protein of Chlamydomonas-Specific family B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCGTGTGCTTAGTAGGCGCCCGCGTCAAGGTGGCTGGGTTGATAACG |
| Internal bar code: | AAGTACGGTGTAAGGTATGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2394 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCTTTACAGTGCGACTCT |
| Suggested primer 2: | AGGTGGCTGGGTTGATAACG |