| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.065757 |
| Chromosome: | chromosome 11 |
| Location: | 559096 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467603 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGACCTACTGCACGTGCGCAGTGGCGTTGGACTCACGCAGTGCGCTGC |
| Internal bar code: | TCAGGTATAAAAACCCAGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2057 |
| LEAP-Seq percent confirming: | 70.9677 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAACACTTGTCCGGTTGGC |
| Suggested primer 2: | TTCGGGAAAATGGAGTCCGG |