| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.065773 |
| Chromosome: | chromosome 16 |
| Location: | 6675366 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673400 | Chlamydomonas-specific family W protein; (1 of 6) PF06701 - Mib_herc2 (MIB_HERC2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGCAGCAGCCAAGTCGAATGTATTGCACTTGCTTGGCTCAAACGGGAT |
| Internal bar code: | GCCCTTAGGAATTGTAGGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2763 |
| LEAP-Seq percent confirming: | 12.5 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACCAAGCCTGATCCAAGT |
| Suggested primer 2: | AAGACACGTGCGCTTCCTAA |