Insertion junction: CLIP2.065774_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.065774
Chromosome:chromosome 3
Location:2588363
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g160200 P4H12,PHX7,PFH12,Cr-P4H-1 (1 of 2) IPR003582//IPR005123//IPR006620//IPR027450 - ShKT domain // Oxoglutarate/iron-dependent dioxygenase // Prolyl 4-hydroxylase, alpha subunit // Alpha-ketoglutarate-dependent dioxygenase AlkB-like; Prolyl 4-hydroxylase 12 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTTTTGTGCAACACGTGCAACACGCCATTACAACTATCCCCCTCCAGA
Internal bar code:CTCCCGCTACAGAAGCATTTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:4987
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:91
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:91

Suggested primers for confirmation by PCR

Suggested primer 1:TGCCGGTTGAGGTACTTGTC
Suggested primer 2:TTTGCGTGCCACGTACTTTG