Insertion junction: CLIP2.065792_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.065792
Chromosome:chromosome 2
Location:2580276
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g092900 GUA1 (1 of 1) 6.3.5.2 - GMP synthase (glutamine-hydrolyzing) / GMP synthetase (glutamine-hydrolyzing); GMP synthetase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATGCTGTAGCTCAGGGTTGCACAGTACACGTCCCCTCTTCCAAAAATGC
Internal bar code:CTCACAACCTCTATCATATAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1896
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGCTTTCAGGCGTTGAAG
Suggested primer 2:GTTGCAGCTCCAGAGGGATT