| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.065812 |
| Chromosome: | chromosome 6 |
| Location: | 7653479 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g302100 | (1 of 1) PTHR12907//PTHR12907:SF10 - EGL NINE HOMOLOG-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGACTTGCGGGCCGGCCTAGGGCCCGGCCCAGGCCCGCCGCCCTCACC |
| Internal bar code: | TCGGGTATGGTCCCGTCGCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 125 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTCATAACCGACCCTAGC |
| Suggested primer 2: | ACCAGACCGTGCTTCAAACA |