| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.065819 |
| Chromosome: | chromosome 10 |
| Location: | 5478684 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g457600 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTTGAAGCTGGACTCTTGCCAGGACTCTTTCCAGGGTTATCGTCGGGC |
| Internal bar code: | TGGGGGGGCGAGGTGGTGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2433 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCACGATGTTGGAGTCCT |
| Suggested primer 2: | AGTCCATGCCCACATCAGTG |