| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.065821 |
| Chromosome: | chromosome 2 |
| Location: | 7491014 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392579 | MRPL115,OPR10,mL115 | (1 of 239) IPR016024 - Armadillo-type fold; mitochondrial ribosome protein mL115, Chlamydomonas-specific | 3'UTR |
| Cre09.g392616 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGTGTCATGTCAGGGCAGGAACGTAGCACGTTTTGGGGCTCTAACCG |
| Internal bar code: | TACTTGGAGGGTATGACAGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 934 |
| LEAP-Seq percent confirming: | 38.0952 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTATTGGAGGGGTTTGGGG |
| Suggested primer 2: | ATTGGGGCTGTCAGATGTGG |