Insertion junction: CLIP2.065839_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.065839
Chromosome:chromosome 11
Location:3591649
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g478050 (1 of 12) IPR000104//IPR002893 - Antifreeze protein, type I // Zinc finger, MYND-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTATAAGGCCTGACTACACCGTTGACAGCACGTACCGTATGGCACATCT
Internal bar code:CTGGTCTTGCGCTGAGGGGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:651
LEAP-Seq percent confirming:96.6667
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTAAAACGAGTGTGGGGG
Suggested primer 2:CTTGGCTTGAACTTTCGGCC