Insertion junction: CLIP2.065840_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.065840
Chromosome:chromosome 8
Location:647980
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g360200 DUR3,DUR3A Urea active transporter; (1 of 3) PTHR11819:SF94 - SODIUM-DEPENDENT MULTIVITAMIN TRANSPORTER-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTCTCGACGCTTCACTCAACCGAATGCGCGGGCAAATCGTCGCGTCAC
Internal bar code:GCGGTAAGACTTGGATTAGTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1567
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:55
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:55

Suggested primers for confirmation by PCR

Suggested primer 1:TCGATCGCATCTAGGTTGGC
Suggested primer 2:GTACTGGCGGTGTGTCTTCA