| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.065844 |
| Chromosome: | chromosome 15 |
| Location: | 343777 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g635400 | ZYS3,ZYS3-1 | (1 of 3) PTHR24193//PTHR24193:SF87 - ANKYRIN REPEAT PROTEIN // SUBFAMILY NOT NAMED; Zygote-specific protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTCCCTCATCCCATCTAACGGCTCCCCCACGCCCCCTGCTCGCCCTC |
| Internal bar code: | AAAAAGGGCGATGGGACGCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 550 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGTCTCCACGTTGAAGAA |
| Suggested primer 2: | CCTCCTCATCCCCTCCTTCA |