| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.065848 |
| Chromosome: | chromosome 12 |
| Location: | 43263 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g483550 | VPE1 | (1 of 1) K01369 - legumain (LGMN); Vacuolar processing enzyme | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCAGGGTCGGTCGTCGGGGCTGGGGGTCCCCAAGGTTTCGTCACGCGG |
| Internal bar code: | CGTGTACCCGAAGGTAAATATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2572 |
| LEAP-Seq percent confirming: | 73.9726 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACTTCAGGGTCGGTCGTC |
| Suggested primer 2: | GCTGTTGAGAACTGTGCTGC |