| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.065891 |
| Chromosome: | chromosome 10 |
| Location: | 5887853 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g460700 | (1 of 15) PTHR12291:SF23 - PROTEIN MEC-15 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGACAGCACGGGCAGGACTTCCGGGACGGACGGAGTAAGGTAGAGCCGC |
| Internal bar code: | TTCAGAAAGTGATCAGTTCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3181 |
| LEAP-Seq percent confirming: | 58.5366 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTGCGGTTTGCTGTACGT |
| Suggested primer 2: | GGCTATCGGGATGGTGATGG |