| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.065896 |
| Chromosome: | chromosome 7 |
| Location: | 5804498 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g353450 | ACS3 | Acetyl-CoA synthetase/ligase; (1 of 3) K01895 - acetyl-CoA synthetase (ACSS, acs) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGCGCAAAAGCGGGCTACCGCGGCACATGGCAAACTTGGTACACCTTT |
| Internal bar code: | CCGAAGGTATGATAGTACGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3450 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGAGGTGCAGCCCTGAGAT |
| Suggested primer 2: | ATATAAAGCGGCGGGGTCAG |