| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.065901 |
| Chromosome: | chromosome 5 |
| Location: | 1152156 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g248450 | CAH5 | Mitochondrial carbonic anhydrase; (1 of 3) PTHR11002//PTHR11002:SF17 - CARBONIC ANHYDRASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAATCCGACTCTTGAGGTCAGGGCGCCTGAGAGCTATCTACACTCGGG |
| Internal bar code: | CCTTCCCGTCAATACGGAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2596 |
| LEAP-Seq percent confirming: | 93.8144 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 97 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGCCGAGAACGTCAAGGT |
| Suggested primer 2: | GCCCACGATATGTTGCCAAC |