| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.065907 |
| Chromosome: | chromosome 9 |
| Location: | 6332627 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g414200 | VPS26 | (1 of 1) K18466 - vacuolar protein sorting-associated protein 26 (VPS26); Subunit of Retromer complex | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCAGGAACGGGGAAGAGCGGTCTCGTTCTACTCGTGGCTCATACATGC |
| Internal bar code: | ATTTTGGGCCGCACCGGGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4705 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGATAGCCTCAGCGCCTG |
| Suggested primer 2: | CTCTAACGCTAGCTGGGAGC |