| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.065908 |
| Chromosome: | chromosome 8 |
| Location: | 4358945 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g384864 | (1 of 1) PF00018//PF14604 - SH3 domain (SH3_1) // Variant SH3 domain (SH3_9) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTCGGCCCACTACTTGGTTCAGGTTACAGACGCGCTTGTTTGGGAGTT |
| Internal bar code: | TGGGTCCAGAGGTTATCGATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1925 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGTTGCTGAGATGGAGGG |
| Suggested primer 2: | CACAAGCACACGTGTCACAG |