| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.065913 |
| Chromosome: | chromosome 2 |
| Location: | 6756573 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387726 | AST1 | Aspartate aminotransferase; (1 of 1) K14454 - aspartate aminotransferase, cytoplasmic (GOT1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTGACTGGGTTTGGGTGCAAGGGGGTGGAAAGGGAGGGAGGCACGATT |
| Internal bar code: | ACTCATAGCGCCGCCTCATAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1127 |
| LEAP-Seq percent confirming: | 3.125 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGAGTACATGGGGCGAA |
| Suggested primer 2: | TTTGACAGCGCATACCAGGT |