| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.065916 |
| Chromosome: | plastome |
| Location: | 61358 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802289 | ChreCp025,rps14,2716974 | (1 of 1) K02954 - small subunit ribosomal protein S14 (RP-S14, MRPS14, rpsN); 30S ribosomal protein S14 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAATATTTATATTCCCGTAAGGGGACGTCCTGCCAACTGCCTCTTTGAA |
| Internal bar code: | CTGCGCCTTTCCGTGGGGTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1906 |
| LEAP-Seq percent confirming: | 81.25 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAACCTGTGTCCAACCAA |
| Suggested primer 2: | TAGCGCTTAGACGAGGACCT |