| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.065924 |
| Chromosome: | chromosome 9 |
| Location: | 2539621 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392750 | (1 of 1) PTHR10972:SF45 - OXYSTEROL-BINDING PROTEIN 9 | outside_mRNA | |
| Cre09.g392800 | POB24 | Proteome of basal body 24 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAAGGCTAAGGATGGGACTGTGACATTAATGGAGGTGGGATGTGGCAG |
| Internal bar code: | TGGTGCTTCGGTCTAAACCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2387 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGGCTGGTGGTATTGGTC |
| Suggested primer 2: | TGAAGGACGAGATTGCGGAC |