| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.065928 |
| Chromosome: | chromosome 13 |
| Location: | 56788 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g562100 | 3'UTR | ||
| Cre13.g562150 | (1 of 1) PTHR23305:SF12 - GTP-BINDING PROTEIN-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTATGGCGTACGGAGTTACATCCACTGGAATGCATCAACGACATCATC |
| Internal bar code: | ATGAGGTTGCTTCGTCTAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1655 |
| LEAP-Seq percent confirming: | 84.6154 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGCAAGGAGTACGTTGTG |
| Suggested primer 2: | TTGATTGCGTGGTGTTGCAG |