Insertion junction: CLIP2.065941_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.065941
Chromosome:chromosome 5
Location:422029
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre05.g243802 (1 of 11) PF07173 - Protein of unknown function (DUF1399) (DUF1399) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTATTGCTCATGTTGCTTCTCTCACAACGTCCCACATCATTGATGCTGCG
Internal bar code:GGCACCTCGCTGGGGAGTTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:797
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTCTGTGGCAGTAGAGCA
Suggested primer 2:GGTCTGCTGCAAAGACTCCT