| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.065965 |
| Chromosome: | chromosome 16 |
| Location: | 854985 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g647750 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCAAGATGTCTACGATCATTTCTATTGAGCAATTCAAGCAGCTTTTGG |
| Internal bar code: | GGTGGGAGGTGTTATGAAGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3626 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCTATGTTTTGCCGACGC |
| Suggested primer 2: | GTAGTCTGCGAGATTCGGGG |