| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.065984 |
| Chromosome: | chromosome 13 |
| Location: | 4179537 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g591400 | COA1,COAA1 | (1 of 1) 2.7.1.33 - Pantothenate kinase / Pantothenic acid kinase; Pantothenate kinase, CoA biosynthesis | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTGTTTTGAGGCAGCGCGTGGTGCGGTGGCTAAGGCCGGGTGGACTA |
| Internal bar code: | ATTCGAAGCTGACGATGACTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 235 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTGCGATTGGTAGGCACG |
| Suggested primer 2: | AACTTCTCCACCCAGCTGAC |