| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.065992 |
| Chromosome: | chromosome 13 |
| Location: | 481954 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g565050 | (1 of 11) PF07173 - Protein of unknown function (DUF1399) (DUF1399) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCTTCGCGCCCCCTACTTCAGCCACTCCTGGCTGGCCGCCGGCGTGCG |
| Internal bar code: | ACACGCAGAAAAGAGTTTAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 77 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACGTGAGGACAGGAGAC |
| Suggested primer 2: | TGCGTTGTTCAAGTGCGAAG |