| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.066002 |
| Chromosome: | chromosome 6 |
| Location: | 8021636 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304850 | (1 of 1) PF04424 - Protein of unknown function (DUF544) (DUF544) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCACGCAAGAGAACGGCGGAGCTGGAGACAAGAAAGGAAGCGAGGCCG |
| Internal bar code: | TCTAACGCCTAATCGTATGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 415 |
| LEAP-Seq percent confirming: | 56.25 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGGTAATGGTGGCCAAG |
| Suggested primer 2: | AAGCACCCTGACACCCAAAA |