Insertion junction: CLIP2.066019_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.066019
Chromosome:chromosome 12
Location:4890114
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre12.g524000 SUC1,GTR7 (1 of 1) 2.4.1.14 - Sucrose-phosphate synthase / UDP-glucose-fructose-phosphate glucosyltransferase; Sucrose-phosphate synthase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGGGGAGCCGGCGGCGGCGGCGAACACCGACGTCGGCGGGGTGCCCGGC
Internal bar code:AACTATTGCTTTTGTATTTTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:324
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CATACACGCATACGCAAGGC
Suggested primer 2:CGTATAAGTGTGTGTGCGCG