| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066022 |
| Chromosome: | chromosome 2 |
| Location: | 7783227 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g146600 | AP4M4 | Mu4-Adaptin; (1 of 1) K12402 - AP-4 complex subunit mu-1 (AP4M1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTAACCTGTTTGCCGCATGAAACTACATCGCCAGCTTCCAAATTGACA |
| Internal bar code: | GAGCAATTTTTCCGTAAATTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5106 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 122 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 122 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCGCCAGGAGAGCTTATC |
| Suggested primer 2: | CGAACACACGAAACACACCC |