| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066024 |
| Chromosome: | chromosome 17 |
| Location: | 3733726 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g726300 | uS14m,MRPS14 | Mitochondrial ribosomal protein S14; (1 of 3) PF00253 - Ribosomal protein S14p/S29e (Ribosomal_S14) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGCAAGAGGCAAAGTCATGCCGCGTATTGCGCCTGCGCCTCAGAGGG |
| Internal bar code: | GGTGTGACGTAGTTTTGACGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1137 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTCCAACCGTTGTTCG |
| Suggested primer 2: | GATGGATGAGGGATGCGAGG |