Insertion junction: CLIP2.066027_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.066027
Chromosome:chromosome 16
Location:913631
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g648300 PHT6,PHT4-6,PHT4F Sodium-dependent phosphate transporter, major facilitator superfamily; (1 of 3) PTHR11662:SF243 - ANION TRANSPORTER 6, CHLOROPLASTIC-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAGCAGTCCTCGGACGCAACCGCGCTTGACCCTTATTGTCAGGGGCTCG
Internal bar code:AGACTTAGTCCGGTCTATGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1074
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACAACCACCGACAAGCGTAT
Suggested primer 2:AGCGTGCAACCTACAGTACC