Insertion junction: CLIP2.066028_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.066028
Chromosome:chromosome 7
Location:497141
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre07.g315750 (1 of 1) 1.1.1.287 - D-arabinitol dehydrogenase (NADP(+)) / NADP(+)-dependent D-arabinitol dehydrogenase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGGAGATTACTGATACTGCATGCGTGCCGGTTTCGACTATGACTGTAGA
Internal bar code:TACAGGTTCAGGTTAGGGACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:70
LEAP-Seq percent confirming:9.09091
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:TCGACTCTCCGACAAGTTGC
Suggested primer 2:CTGTTGCGGTGTTGTGACTG