| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.066037 |
| Chromosome: | chromosome 6 |
| Location: | 2951777 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273413 | (1 of 1) K14835 - ribosomal RNA methyltransferase Nop2 [EC:2.1.1.-] (NOP2) | 3'UTR | |
| Cre06.g273450 | POB11 | Proteome of basal body 11 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGTGCTAGGCCCCGTGTACACGCCCGCTTGCGCAATCGCTCGCGGTA |
| Internal bar code: | ACTTGGGGACCAATAGTTTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2930 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 83 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCGGACTGAAGCTCGATC |
| Suggested primer 2: | CACCATAAGCACCGCAATCG |