Insertion junction: CLIP2.066038_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.066038
Chromosome:chromosome 13
Location:4170794
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g591350 MFT27 Major facilitator superfamily transporter; (1 of 22) IPR011701//IPR020846 - Major facilitator superfamily // Major facilitator superfamily domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACCCACCGCCAACATTACTTTGTTGTTATGTCATATCACATTCGTTT
Internal bar code:GATACTATACCCCATGTAAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1254
LEAP-Seq percent confirming:93.5897
LEAP-Seq n confirming:73
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:78

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGTGATCCAGGTGCACAT
Suggested primer 2:TTGATTTGCTGTGTGGCTGC