Insertion junction: CLIP2.066041_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.066041
Chromosome:chromosome 8
Location:3411941
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre08.g378150 GLD2 Glucose-6-phosphate dehydrogenase; (1 of 2) 1.1.1.49 - Glucose-6-phosphate dehydrogenase (NADP(+)) / GPD intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATTATATGAGAAGTGAAGGTAAAGTCCGGAACAGCAGTATAGGGGGGGG
Internal bar code:TCACCACAGAGATAGGGTATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:537
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AGAACCACCTGCTGCAGATC
Suggested primer 2:CACCTGAACTGCACTCGGAT